Characterization of <I>Colletotrichum gloeosporioides</I> Resistant to Carbendazim
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Abstract
Isolates of Colletotrichum gloeosporioides causing anthracnose disease were obtained from infected mango fruits collected from fresh market in Chiang Mai. One hundred isolates were successfully isolated. The carbendazim-resistant assay was conducted on potato dextrose agar amended with carbendazim at various concentrations: 0.1, 1, 10, 100, 500 and 1,000 mg/l, respectively. These isolates were classified into four representative phenotypes of reactions as highly resistant (HR; ³ 500 mg/l), moderately resistant (MR; £ 100 mg/l), weakly resistant (WR; £ 10 mg/l) and sensitive (S; £ 1 mg/l). The results showed that 95 isolates were HR, and 5 isolates were S phenotype. The differences in the carbendazim-resistant phenotypes were conspicuous in sequence analysis of the partial second beta-tubulin (TUB2) gene. Amino acid sequence analysis in comparison with wild type C. gloeosporioides f. sp. aeschynomene (accession no. U14138) was carried out. HR phenotype revealed a substation of codon 198, which encoded glutamic acid (E) in S phenotype, was converted to a codon for alanine (A) which was closely associated with conferring carbendazim resistance of phenotypic mutation from the field.
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References
Akem, C. N. 2006. Mango anthracnose disease: present status and future research priorities. Plant Pathol. J. 5: 266-273.
Buhr, T. L., and M. B., Dickman. 1994. Isolation, characterization, and expression of a second β-tubulin-encoding gene from Colletotrichum gloeosporioides f. sp. aeschynomene. Appl. and Environ. Microbiol. 60: 4155-4159.
Chung, W. H., H. Ishii, K. Nishimura, M. Fukaya, K. Yano, and Y. Kajitani. 2006. Fungicide sensitivity and phylogenetic relationship of anthracnose fungi isolated from various fruit crops in Japan. Plant Dis. 90: 506-512.
Damicone, J., and D. Smith. 2009. Fungicide resistance management. (On-line). Oklahoma Cooperative Extension Fact Sheets. Available: http://pods.dasnr. okstate.edu/docushare/dsweb/Get/Document-2317/F-7663web.pdf (July 1, 2010).
Deising, H. B., S. Reimann, and S. F. Pascholati. 2008. Mechanisms and significance of fungicide resistance. Brazil. J. Microbiol. 39: 286-295.
Kim, Y. S., J. Y. Min, B. K. Kang, N. V. Bach, W. B. Choi, E. W. Park, and H. T. Kim. 2007. Analyses of the less benzimidazole-sensitivity of the isolates of Colletotrichum spp. causing the anthracnose in pepper and strawberry. Plant Pathol. J. 23: 187-192.
Koenraadt, H., S. C. Somerville, and A. L. Jones. 1992. Characterization of mutations in the beta-tubulin gene of benomyl-resistant field isolates of Venturia inaequqlis and other plant pathogenic fungi. Phytopathology 82: 1348-1354.
Kumar, A. S., N. P. E. Reddy, K. H. Reddy, and M. C. Devi. 2007. Evaluation of fungicidal resistance among Colletotrichum gloeosporioides isolates causing mango anthracnose in Agri Export Zone of Andhra Pradesh, India. Plant Pathol. Bull. 16: 157-160.
Ma, Z., and T. J. Michailides. 2005. Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopathogenic fungi. Crop Protection 24: 853-863.
Maymon, M., A. Zveibil, S. Pivonia, D. Minz, and S. Freeman. 2006. Identification and characterization of benomyl-resistant and sensitive populations of Colletotrichum gloeosporioides from statice (Limonium spp.). Phytopathology 96: 542-548.
Peres, N. A. R., N. L. Souza, T. L. Peever, and L. W. Timmer. 2004. Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus. Plant Dis. 88: 125-130.
Prabakar, K., T. Raguchander, D. Saravanakumar, P. Muthulakshmi, V. K. Parthiban, and V. Prakasam. 2008. Management of postharvest disease of mango anthracnose incited by Colletotrichum gloeosporioides. Arch. Phytopathology Plant Protect. 41: 333-339
Ru-Lin, Z., and H. Jun-Sheng. 2007. Cloning of a carbendazim-resistant gene from. Colletotrichum gloeosporioides of mango in South China. Afr. J. Biotechnol. 6: 143-147.
Singh, S. B., I. Mukherjee, J. Maisnam, P. Kumar, M. Gopal, and G. Kulshrestha. 2008. Determination of pesticide residues in IPM and non-IPM sample of mango (Mangifera indica). J. Environ. Sci. Health Part B. 43: 300-306.
Sutton, B. C. 1992. The genus Glomerella and its anamorph Colletotrichum. Pp. 1 – 27. In: Colletotrichum: Biology, Pathology and Control. J. A. Bailey, and M. J. Jeger, eds. CAB International, Wallingford, UK