Isolation and Identification of Microorganisms for Natural Strobilanthes sp. Dye Production

Authors

  • พิชญาพร ภิญญ์ญาพัช Biotechnology Program, Maejo University-Phrae Campus
  • ธวัชชัย ชัยธวัชวิถี Biotechnology Program, Maejo University-Phrae Campus
  • เอกอาทิตย์ ฤทธิเดชยิ่ง Biotechnology Program, Maejo University-Phrae Campus

Keywords:

indigo dye, Strobilanthes sp., isolation, identification, microorganis

Abstract

Two hundreds and sixty five isolations of microorganisms were obtained from 240 samples of fermentation liquor during indigo fermentation processes from indigo fermentation plant in Ban Nakuha, Meung district, phrae province. Only 160 isolations could change indigo blue color into leuco-indigo. Of these, there were 6 isolations showed the highest leuco-indigo production activity; L4NG3(-2)I1 L1P1(-3)I1, L4NG3(-2)I3, L4NG4(-3)I2, L2P2(-2)I2 and L1P4(-2)I3. From the morphological study; L1P1(-3)I1 L2P2(-2)I2, L1P4(-2)I3 and L4NG4(-3)I2 were found in rod shape, whereas L4NG3(-2)I1 and L4NG3(-2)I3 were found in oval. After the biochemical tests, the results showed that L4NG3(-2)I1, L1P4(-2)I3 L4NG3(-2)I3 were in the Genus Bacillus, L4NG4(-3)I2 was in the Genus Enterobacteriaceae, whereas L1P1(-3)I1 and L2P2(-2)I2 could not be identified. The 16S rDNA sequences of L4NG3(-2)I1, L2P2(-2)I2 L1P1(-3)I1, L1P4 (-2)I3, L4NG3(-2)I1 and L4NG4(-3)I2 displayed high identity to Bacillus sp., Streptomyces sp. Bacillus sp., B. flexus, B. aryabhattai, and B. subtilis, respectively.

Author Biographies

พิชญาพร ภิญญ์ญาพัช, Biotechnology Program, Maejo University-Phrae Campus

Biotechnology Program, Maejo University-Phrae Campus

ธวัชชัย ชัยธวัชวิถี, Biotechnology Program, Maejo University-Phrae Campus

Biotechnology Program, Maejo University-Phrae Campus

เอกอาทิตย์ ฤทธิเดชยิ่ง, Biotechnology Program, Maejo University-Phrae Campus

Biotechnology Program, Maejo University-Phrae Campus

Published

2018-05-11

How to Cite

ภิญญ์ญาพัช พ., ชัยธวัชวิถี ธ., & ฤทธิเดชยิ่ง เ. (2018). Isolation and Identification of Microorganisms for Natural Strobilanthes sp. Dye Production. Journal of Agricultural Research and Extension, 35(1), 45–54. retrieved from https://li01.tci-thaijo.org/index.php/MJUJN/article/view/192599

Issue

Vol. 35 No. 1 (2018): January-April 2018

Section

Research Article

License

This article is published under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0), which allows others to share the article with proper attribution to the authors and prohibits commercial use or modification. For any other reuse or republication, permission from the journal and the authors is required.