Inhibition of Free Radicals and Matrix Metalloproteinase of Virgin Coconut Oil and Its Reduction of Inflammatory Product
Keywords:
Virgin coconut oil, , Antioxidant activity, Anti-matrix metalloproteinase activity, Pain relief productAbstract
The virgin coconut oil (VCO) is extracted from fresh and mature coconut meat (Cocos nucifera Linn.) without using chemical reagent or heating in process. The VCO is colorless and clear, it has the aroma of fresh coconut. The present study was carried out to determine VCO for phenolic contents, antioxidant, matrix metalloproteinase inhibitory activities, and then to formulate VCO pain relief product and evaluated their stability of the product. The results revealed that the phenolic contents of VCO was 14.79±0.19 mg GAE. The VCO presented DPPH and superoxide radical scavenging activities with GAE value of 0.56±0.01 mg and 36.32±0.75 mg, respectively. Additionally, matrix metalloproteinase inhibitory activity can be reduced inflammation and protected degradation of cartilage which VCO had the highest inhibitory effect against MMP-9 as 84.53±1.00%. The pain relief ointment product has been developed using VCO as active ingredient. The stability of formulation was tested with subjecting samples at room temperature, 4 and 45⸰C for 45 days and heating-cooling cycles at intervals of 24 hours over 5 cycles. The product demonstrated good physical stability under the various conditions. The results of product satisfaction revealed that highly satisfied with the VCO ointment and no skin irritation in 20 volunteers.
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