Effect of Heat Stress on Survival Rate and Gene Expression in Fibroblast Cells of Khao Lamphum and Crossbred Khao Lamphun Cattle
Keywords:
gene expression, hyperthermia, cell fibroblast, crossbreed cattle, Khao Lamphum cattleAbstract
The study aimed to determine the survival rate and gene expression in fibroblast cells from Khao Lamphum, crossbreed Khao Lamphun-Angus and Khao Lamphun-Charolais cattle under heat stress. The fibroblast cells were collected from 9 female cows' ears and then were cultured at a temperature of 38.5°C. Study 1: cell survival rate of fibroblast cells were cultured under high temperature conditions at 42°C for 6, 24 and 48 hours. Result showed survival rate of Khao Lamphun cattle (n=3) were 99.59, 98.73 and 92.88%, respectively, which was higher than crossbreed Khao Lamphun-Angus (n=3) as 98.73, 95.70 and 88.61%, respectively and Khao Lamphun-Charolais (n=3) as 99.53, 96.36 and 88.85%, respectively (P<0.05). The relation between duration and survival rate were related in positive direction Khao Lamphum (0.896), Khao Lamphun-Angus (0.647) and Lamphun-Charolais (0.862), respectively. Study 2: gene expression in heat tolerance (Bcl-2, HSP90 and HSP70) after fibroblast cells exposed to heat stress at 42°C at 6, 12, and 24 hours. The mRNA expression levels were evaluated using the RT-PCR method measurable concentrations of Bcl-2, HSP90 and HSP70. Bcl-2, HSP90 and HSP70 gene were all expressed when cultured under high temperature conditions at 42°C. In conclusions, survival rate of fibroblast cell under high temperature conditions the Khao Lamphun was higher than of crossbred cattle. When cell exposed to high temperature cell expressed of gene that related to heat tolerance, which allows the crossbred cattle adapting to the tropical environment of Thailand.
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