Prevalence of Ehrlichia canis, Hepatozoon canis, Babesia canis Anaplasma platys and Trypanosoma evansi Infections in Dogs in Chiang Mai

Authors

  • Wasin Charerntantanakul Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai
  • Pennapa Takam Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai
  • Wilawan Ruansit Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai
  • Dante Fabros Jr. Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai
  • Pichanan Suebsaard Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai
  • Onanong Seng-Lao Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai
  • Thipkamon Thikarach Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai
  • Worapat Prachasilchai Department of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai University Chiang Mai
  • Chollada Sodarat Department of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai University Chiang Mai
  • Phacharaporn Tadee Faculty of Animal Science and Technology, Maejo University, Chiang Mai,
  • ไพโรจน์ พงศ์กิดาการ Faculty of Animal Science and Technology, Maejo University, Chiang Mai,
  • Pattama Charerntantanakul Pat Veterinary Clinic, Chiang Mai
  • Runjuan Isararuk Faculty of Agricultural Technology, Songkhla Rajabhat University, Songkhla
  • Reankeaw Praphet Institute of Product Quality and Standardization, Maejo University, Chiang Mai,

Keywords:

prevalence, blood parasites, dog, Chiang Mai, multiplex polymerase chain reaction

Abstract

         

This study determined the prevalence of blood parasite infections in dogs in Chiang Mai, using multiplex polymerase chain reaction (PCR) developed in our laboratory. It also generated database of blood parasite infections in dogs for launching veterinary services of Faculty of Veterinary Medicine, Maejo University. Blood was collected from 870 dogs in 19 districts. Multiplex PCR was conducted to detect VirB9 gene of Ehrlichia canis, 18S rRNA gene of Hepatozoon canis and Babesia canis, and groEl gene of Anaplasma platys. Single PCR was used to detect VSG gene of Trypanosoma evansi. The results showed that dogs had the highest prevalence for H. canis infection (331 dogs: 56.7%), followed by        E. canis (6 dogs: 1%), A. platys (5 dogs: 0.9%), and B. canis (2 dogs: 0.3%), respectively. Some dogs had co-infections with two or more parasites, which were H. canis + B. canis (191 dogs: 32.7%), followed by E. Canis + H. canis (15 dogs: 2.6%), H. canis + A. platys (12 dogs: 2.1%), E. canis + H. canis + B. canis (8 dogs; 1.4%), H. canis + B. canis + A. platys (8 dogs: 1.4%), E. canis + H. canis + A. platys (2 dogs: 0.3%), and  E. canis + H. canis + B. canis + A. platys (4 dogs: 0.7%). No T. evansi infection was found.  There was no association between blood parasite infections and gender and age range of dogs. There was a significant association between low blood parasite infection and availability of veterinary services within the 10 kilometers from the dog residences. 

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Published

2022-08-22

How to Cite

Charerntantanakul, W., Takam, P. ., Ruansit, W. ., Fabros Jr., D. ., Suebsaard, P., Seng-Lao, O. ., Thikarach, T. ., Prachasilchai, W. ., Sodarat, C. ., Tadee, P. ., พงศ์กิดาการ ไ. ., Charerntantanakul, P. ., Isararuk, R., & Praphet, R. . (2022). Prevalence of Ehrlichia canis, Hepatozoon canis, Babesia canis Anaplasma platys and Trypanosoma evansi Infections in Dogs in Chiang Mai. Journal of Agricultural Research and Extension, 39(2), 105–115. retrieved from https://li01.tci-thaijo.org/index.php/MJUJN/article/view/242667

Issue

Vol. 39 No. 2 (2022): May - August 2022

Section

Research Article

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