Isolation and Selection of Bacteria Capable of Extracting Chitosan from Shrimp Shell Waste

Authors

  • Nuttaporn Chanchay Agro-Industrial Biotechnology, Maejo University Phrae Campus, Phrae
  • Phorndara Sritong Agro-Industrial Biotechnology, Maejo University Phrae Campus, Phrae
  • Ansaya Boonprajaub Agro-Industrial Biotechnology, Maejo University Phrae Campus, Phrae

Keywords:

chitosan, chitin, bacteria, isolation, shrimp shell

Abstract

Isolation and selection of bacteria capable of extracting chitosan from shrimp shell. Three isolates of microorganisms were found; PC-08 of chitosan liquid fertilizer, NC-04 of chitosan ferment from shrimp shells and SC-08 of shrimp shell chitosan ferment from Thai style barbecue restaurant. Then run the test in MMCT, it was found that NC-04 isolate had the best enzyme activity of 16.746±0.019 units/mg/min. After that, compared with chemical methods, it was found that the chitosan production digested with sodium hydroxide had the higher removal values of the acetyl group, water solubility, percentage yield of chitosan, and pH than method for bacterial separation from shrimp shell. The isolated bacteria were analyzed for morphological characteristics and found that NC-04 isolate was morphologically consistent with 99% Acinetobacter baumannii. However, the method of bacterial isolation and selection of bacteria for extraction of chitosan from shrimp shells still need to be developed on an industrial scale.

References

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Published

2023-04-22

How to Cite

Chanchay, N. ., Sritong, P. ., & Boonprajaub, A. . (2023). Isolation and Selection of Bacteria Capable of Extracting Chitosan from Shrimp Shell Waste. Journal of Agricultural Research and Extension, 40(1), 104–115. retrieved from https://li01.tci-thaijo.org/index.php/MJUJN/article/view/243327

Issue

Vol. 40 No. 1 (2023): January-April 2023

Section

Research Article

License

Copyright (c) 2023 Journal of Agricultural Research and Extension

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