Optimal Concentrations of Tannin Extracted from Terminalia catappa Leaves on Tail Regeneration of Siamese Fighting Fish (Betta splendens)
Keywords:
tannin, Terminalia catappa, tail regeneration, concentration, Siamese fighting fishAbstract
The objective of this research was to study the optimal concentration of tannin extract from Terminalia catappa leaves on tail regeneration of Siamese fighting fish (Betta splendens). The experiment was divided into two experiments. The first experiment was performed the optimal concentration of the tannin extract from Terminalia catappa leaves on tail regeneration in the Siamese fighting fish. The caudal fin of the Siamese fighting fish was cut off and cultured in the tannin extract from Terminalia catappa leaves at the concentration of 0, 5, 10, 20 and 40 ppm. Treatment with each concentration was repeated 3 times over 5 weeks. The results showed that the Siamese fighting fish soaked in 40 ppm tannin extract had the fastest tail regeneration rate (p<0.05) comparing to the other experiments. The second experiment was to investigate the optimal concentration of the tannin extract from Terminalia catappa leaves at the concentration of 0, 5, 10, 20 and 40 ppm in combination with 1 percent sodium chloride (NaCl). This experiment was repeated 3 times. The results revealed that the combination of 1 percent sodium chloride and tannin extract from Terminalia catappa leaves 40 ppm showed the highest tail regeneration rate of the Siamese fighting fish (p<0.05) comparing to the control experiments (100 percent within 3 weeks). Therefore, the optimal concentration of the tannin extract from Terminalia catappa leaves resulting in the fastest tail regeneration of the Siamese fighting fish was 40 ppm. Besides, the tannin extract should be combined with 1 percent sodium chloride for the best result.
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