Genetic Variation of Geographical Indication Plant, Nan Golden Orange for Conservation and Genetic Management
Keywords:
genetic variation, geographical indication, Nan Golden Orange, Citrus reticulateAbstract
Nan Golden Orange is a geographic indication plant of Nan province. It is also an important economic crop. Having information on the genetic variation of Nan Golden Orange will be of conservation benefit and genetic management, valuable to plan more proficient breeding programs. The genetic variation was evaluated from 5 groups of Nan Golden Orange leaflets (30 samples in each group), which were collected from 1. original Nan Golden Orange propagate vegetatively (G1) 2. seed grown from original Nan Golden Orange (G2) 3. Nan Golden Orange farm (A) 4. Nan Golden Orange farm (B) 5. Nan Golden Orange farm (C). These samples were investigated by 5 microsatellite loci. The number of alleles per locus (A) ranged from 5.00-5.40, allelic richness (Ar) extended from 4.79-5.07 alleles, observed heterozygosity (Ho) ranged from 0.92-0.97, effective population size (Ne) was small in the sample B. The bottleneck was found in sample G2. Genetic differences (pairwise FST) were found in samples G1, G2, B, and C. Consistent with the dendrogram, sample G1 was different from other samples. The genetic distance was the highest between G1 and B (0.166). The genetic variations in the Nan Golden Orange were the result of management which demonstrated the importance of conservation and genetic management. Understanding good propagation practices for preserving genetic diversity will help maintaining the quality of the Nan Golden Orange in order to be conform with the quality of geographic indication.
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