Genetic Polymorphisms of Chicken Growth Hormone (cGH) Gene in Dang Surat Chicken at PhunPhin District, Suratthani Province
Keywords:
cGH, genetic polymorphism, Dang Suratthani Thai native chickenAbstract
The chicken growth hormone (cGH) gene plays a crucial role in controlling growth and muscle development in chickens. The aim of the study was to evaluate the genetic polymorphism of the cGH gene in the Dang Surat chicken using PCR-RFLP. The cGH gene was genotyped in 172 samples of Dang Surat chicken obtained from Phunphin district, Surat Thani province. Dang Surat chicken is a native chicken that is very popular in southern Thailand. PCR was used to amplify genomic DNA for the cGH gene (429 bp). The PCR product was cut using the EcoRV enzyme. The genotyping of the cGH gene of Dang Surat chicken produced three genotypes, GG, AG, and AA, and two alleles, G (429 bp) and A (296 bp and 133 bp) and the frequency of G was higher than that of A. There were no significant deviations in genotype frequencies from the Hardy-Weinberg equilibrium. The values of expected heterozygosity (He) and observed heterozygosity (Ho) were 0.51 and 0.49, respectively. The study revealed that cGH/ EcoRV gene was polymorphic in Dang Surat chickens that were genotyped. Therefore, the cGH gene may be an important candidate gene for production traits in Thai native chickens and could be used for indigenous chicken utilization and conservation.
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