Effect of Crocodile Oil on Human Keratinocyte Cell Line Viability Antioxidant Activity and Its Application as Tilapia Fish Feed

Authors

  • Doungporn Amornlerdpisan Faculty of Fisheries Technology and Aquatic Resources, Maejo University
  • Metas Ngernjan Faculty of Fisheries Technology and Aquatic Resources, Maejo University
  • Yamaratee Jaisin Faculty of Fisheries Technology and Aquatic Resources, Maejo University
  • Teerawat Rattanaphot Center of Excellence in Agricultural Innovation for Graduate Entrepreneur, Maejo University
  • Kriangsak Mengumphan Center of Excellence in Agricultural Innovation for Graduate Entrepreneur, Maejo University

Keywords:

crocodile oil, cell viability, antioxidant activity, fish feed, growth performance

Abstract

Crocodile fat, a by-product of community enterprise farms, was extracted in from of oil. Crocodile oil (CO) was investigated for its ability to enhance cell viability and antioxidant activity on human keratinocyte line (HaCaT cell) and its ability to increase growth performance of tilapia when used as supplement. From the results, the unsaturated fatty acid content of crocodile oil was 71.26 g/100 g, composing of omega 3, 6, and 9 fatty acids at the amounts of 1.67, 24.53, and 39.71 g/100 g, respectively. In the study on HaCaT cells, when CO was dissolved in tween 20, the concentrations of 0.125 and 0.25% showed no cytotoxicity and also enhanced cell viability. Additionally, CO possessed antioxidant activity via reduction of NO production induced by UVB irradiation. Furthermore, CO was fed to tilapia for over 4 months, and the results on growth performance demonstrated that 4% CO could be used as an ingredient in fish feed. However, need more pharmacological research on CO is needed before it can be used commercially.

 

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Published

2023-04-22

How to Cite

Amornlerdpisan, D. ., Ngernjan, M. ., Jaisin, Y. ., Rattanaphot, T. ., & Mengumphan, K. . (2023). Effect of Crocodile Oil on Human Keratinocyte Cell Line Viability Antioxidant Activity and Its Application as Tilapia Fish Feed. Journal of Agricultural Research and Extension, 40(1), 91–103. retrieved from https://li01.tci-thaijo.org/index.php/MJUJN/article/view/252112

Issue

Vol. 40 No. 1 (2023): January-April 2023

Section

Research Article

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