The Effect of Ascorbic Acid Supplementation in Semen Diluent on Semen Quality at Different Storage Times
Keywords:
ascorbic acid, fresh semen, storage time, semen quality, Thai native chickenAbstract
Effects of ascorbic acid supplementation in semen extenders on fresh and liquid semen quality of Thai native chicken breed were investigated to determine the appropriate levels of supplementation. In the study, four levels of ascorbic acid supplementation were studied at 0, 10, 20 and 30 mM in Schramm diluents. For preservation of the fresh and liquid semen were stored at 4°C for a period of 24, 48 and 72 hours before percentage of mass movement, percentage of progressive motile sperm, sperm concentration and alive sperm were studied. The results showed that different levels of ascorbic acid supplementation had no significant (P>0.05) effects on fresh semen quality, including the mass movement, percentage of progressive motile sperm, percentage of alive sperm and sperm concentration, The storage of diluted semen at 4°C for a period of 24 hours showed a statistically significant difference in mass movement (P<0.05). The supplementation of ascorbic acid 30 mM gave the best score mass movement (3.12±1.41) followed by ascorbic acid 20, 10 and 0 mM, respectively (2.75±2.36, 2.75±2.62 and 2.25±1.15, respectively) but when kept for 48 and 72 hours, it was found that the mass movement showed no significant difference (P>0.05).
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