Acute Toxicity of Ammonia on Tilapia Fingerling (Oreochromis niloticus) Cultured at Different pH Levels
Keywords:
Acute toxicity, ammonia, pH, tilapia fingerlingAbstract
Acute toxicity of ammonia on tilapia fingerling (Oreochromis niloticus) cultured at pH 5, 7 and 9 was studied by using static bioassay methods to determine the median lethal concentration of ammonia that kill haft of tilapia fingerling population within 24 hours (24-hr LC50). Ten tilapia fingerings with average size of 3 cm were placed in a 2.5 l test jar containing 2 l of water. Treatments which included 3 levels of pH 5, 7 and 9. Behavior and mortality rate of tilapia fingerings were observed in this studied within 24 hours. Probit analysis was performed for evaluating the median lethal concentration. The 24-hr LC50 of ammonia at pH 5, 7 and 9 were found to be 2.35 (1.07-5.21), 10.54 (6.34-18.76 and 1.56 (0.72-3.43) ppm respectively. The results from this study showed that different pH levels had an effect on levels of ammonia toxicity and mortality rate of tilapia fingerling. At pH 9, ammonia was most toxic to tilapia fingerling, followed by pH 5 and 7, respectively.
References
American Public Health Association (APHA). 1992. Standard Method for the Examination of Water and Wastwater. 18th. Washington, D.C: American Public Health Association. 1360 p.
Asawaketmanee, N. 2006. Freshwater Fish Culture. Songkhla: Faculty of Agricultural Technology, Songkhla Rajabhat University. 168 p. [in Thai]
Boyd, C.E. 1982. Water Quality Management for Pond Fish Culture. New York: Elsevier Scientific Publishing Company. 318 p.
Boyd, C.E. and C.S. Tucker. 1998. Pond Aquaculture Water Quality Management. Boston: Kluwer Academic Publishers. 700 p.
Chansue N. 2010. Fish Disease: Internal Medicine and Clinical Practice. Bangkok: Holistic Publishing. 691 p. [in Thai]
Chen, J.C. and S.Y. Cheng. 1993. Studies on hemocyanin and haemolymph protein levels of Penaeus japonicus based on sex, size and moulting cycle. Comp. Biochem. Physiol. 106: 293-296.
Chervinski, J. 1982. Environmental Physiology of Tilapia. pp. 119-128. In Pullin, R.S.V. and R.H. Lowe-McConnell. (eds.). The Biology and Culture of Tilapia. In ICLARM Conference Proceedings 7. Manila: International Center for Living Aquatic Resources Management.
Dent, D. 1986. Acid Sulphate Soils: A Baseline for Research and Development. Netherlands: International Institute for Land Reclamation and Improvement. 204 p.
Finney, D.J. 1971. Probit Analysis. 3rd edition. Cambridge: Cambridge University. 331 p.
Hiranwattana, S., B. Sricharoentham and R. Pitipornchai. 1993. Tilapia Cage Culture in Dokgrai Reservoir, Rayong Province. 33 p. In Research Report. Bangkok: Inland Fisheries Research Institute, Department of Fisheries. [in Thai]
Kaewmanee, C. 2009. Substitution of Fish Meal with Plant Protein in Diets on Nursing of Tilapia (Oreochromis spp.) Growth Performance, Ammonia Excretion and Disease Resistance after Transportation. Master Thesis. Kasetsart University. 207 p. [in Thai]
Keratethaweesuk A., O. Jintasataporn and Y. Musig. 2013. Acute Toxicity of Metaldehyde and Acute Toxicity of Ammonia Plus Metaldehyde in Nile Tilapia (Oreochromis niloticus). pp. 449-456. In Proceedings of 51st Kasetsart University Annual Conference, 5-7 February 2013. Bangkok: Kasetsart University. [in Thai]
Kongprasert, C. 2000. Fish Farming. 1st. Bangkok: Kasetsart Book Publishing. 223 p. [in Thai]
Laongsiriwong, N., K. Silpachan and L. Laongsiriwong. 2000. Ammonia and Aquaculture. 8 p. In Research Report. Songkhla: Coastal Aquaculture Research and Development Division. [in Thai]
Limsuwan C. 2000. Thai Shrimp 2000. Bangkok: Charanrat Printing House. 260 p. [in Thai]
Mac Intyre, C.M., T. Ellis, B.P. North and J.F. Turnbull. 2008. The Influences of Water Quality on the Welfare of Farmed Rainbow Trout: A Review; In Fish Welfare. Singapore: Blackwell Publishing Ltd. 299 p.
Nonwachai T. 2012. Effects of Dissolved Oxygen, Ammonia and pH Levels on Feed Intake, Growth, Survival, Non- Specific Immune Characteristic of Pacific White Shrimp (Litopenaeus vannamei) and Challenged with Vibrio harveyi. Doctoral Dissertation. Kasetsart University. 129 p. [in Thai]
Nonwachai T. and S. Wanjing. 2016. Effect of pH levels and ammonia on survival rate of red hybrid tilapia larvae (Oreochromis sp.). Khon Kaen Agriculture Journal 44(1): 644-645. [in Thai]
Prasertsom S. 2004. Acute Toxicity of Ammonia on Sea Bass Fry (Lates calcarifer, Bloch). 19 p. In Research Report. Songkhla: Coastal Aquaculture Research and Development Division. [in Thai]
Robert, V.T., R.C. Russo and C.E. Smith. 1978. Acute toxicity of ammonia and nitrite to cutthroat trout fry. Trans. Am. Fish. Soc. 107(2): 361-368.
Thongdon-A.R. 2013. Study on Diseases and Parasites in Cage-cultured Red Hybrid Tilapia (Oreochromis sp.). Doctoral Dissertation. Kasetsart University. 178 p. [in Thai]
Throp, F.J. and P.S. Lake. 1974. Toxicity bioassays of cadmium and zinc on the freshwater shrimp, Paratya tasmanensis. Marine and Freshwater Research 25: 97-104.
Wajsbrot, N., A. Gasith, M.D. Krom and D.M. Popper. 1991. Acute toxicity of ammonia to juvenile gilthead seabream Sparus aurata under reduced oxygen levels. Aquaculture. 164: 227-288.
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