Effect of Harvest Time and Season on Antioxidant Activity, Anthocyanin and Total Phenolic Content of Butterfly Pea Flower
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Abstract
Butterfly pea (Clitoria ternatea L.) is known for its medicinal properties and as a natural colorant in several countries. Potential and uses of several secondary metabolites extracted from the plant are widely studied; however, improvement of this species for better phytochemical properties is still limited. Phytochemical characterization of butterfly pea germplasm would provide important information for management and utilization of the germplasm for such breeding purpose. Environment may affect phytochemical properties, the matter of which has not been much studied in the species. This study aimed to investigate whether harvest time in a day and season affect phytochemical properties of butterfly pea flowers. Fourteen accessions of butterfly pea germplasm were studied. Their flowers were collected in three different times of the day, i.e., in the morning (8-9 am), in the midday (12-1 pm), and in the afternoon (4-5 pm), in summer (April and May, 2017) and rainy (August, 2017) seasons. The result showed that time in a day affected the ferric reducing antioxidant power (FRAP assay). Flower harvested in the morning had significantly higher FRAP value than those harvested in the midday. Interaction between harvest time of day and harvest season was found affecting DPPH radical scavenging assay (DPPH). The flowers collected in the afternoon in summer had the highest DPPH value, whereas those harvested in the midday in summer had the lowest value. Flowers harvested in rainy season had higher total phenolic content and total anthocyanin content than those harvested in the summer.
Article Details
King Mongkut's Agricultural Journal
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