Survival Rates and Heat Shock 70 Gene Expression in the Fibroblast Cell Lines of 3 Types of Beef Cattle Bred under Hydrothermal Conditions
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Abstract
Gene Heat shock proteins (HSP70) play an important role in the adaptation to survival and heat response of cells. The purpose of this study was to determine the effect of hot temperatures on the survival rates and gene expression of fibroblast cells from Khao Lamphun cattle, crossbreed Khao Lamphun-Angus cattle and Khao Lamphun-Charolais cattle. The fibroblast cells were cultured in a 5% CO2 incubator at 38.5°C. Study 1: The cell survival rate of fibroblast cells was determined after culture under hydrothermal conditions at 42°C for 24 and 48 hours. The cells of Khao Lamphun cattle (97.73±0.16 and 93.33±1.90 percent) showed a viability rate higher than that of crossbreed Khao Lamphun-Angus cattle (96.36±0.35 and 88.88±1.31 percent) and Khao Lamphun-Charolais (96.70±0.44 and 89.16±1.37 percent; p<0.05). Study 2: The HSP70 gene expression of fibroblast cells after exposure to the hot temperature of 42°C for 6, 12 and 24 hours was evaluated using the RT-PCR method. It was found that HSP70 gene was expressed in all groups. This demonstrated the mechanism of cellular heat response of both native and crossbred cattle. In conclusion, the growth rate of fibroblast cells increased during in vitro culture. The survival rate of fibroblast cells under hydrothermal conditions from Khao Lamphun cattle was higher than that of fibroblast cells from crossbred cattle. The fibroblast cells of Khao Lamphun and crossbred cattle showed HSP70 gene expression under hot conditions, which suggests that the fibroblast cells of beef cattle should be able to tolerate heat and adapt to the tropical conditions of Thailand.
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King Mongkut's Agricultural Journal
References
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