Effect of Dietary Melaleuca cajuputi Crude Extract on Growth, Hematology, and Resistance to Bacteria in Common Carp (Cyprinus carpio)
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Abstract
This study aimed to investigate growth performance, hematological parameters, and antibacterial efficacy in carp fed with different levels of Melaleuca cajuputi leaf extract supplementation. The first experimental setup consisted of five different levels of M. cajuputi leaf extract supplementation: 0 (control), 5, 10, 15, and 20 g/kg. After 8 weeks, no significant differences were observed in growth performance between treatment groups (P>0.05). The second experimental setup involved feeding fish with five different levels of M. cajuputi leaf extract: 0 (control), 10, 20, 30, and 40 g/kg. After 2 weeks of feeding, antibacterial efficacy was tested by challenging the fish with pathogenic Aeromonas hydrophila. Seven days post-challenge, fish receiving 20 g/kg extract showed a higher survival rate (80%) compared to the control group without extract supplementation (0%). Hematological parameters in fish fed with 20 g/kg extract supplementation, compared to the control group, showed red blood cell (RBC) counts of 3.34±0.11x106/µL and 1.82±1.58x106/µL, and white blood cell (WBC) counts of 1.83±0.51x103/µL and 1.33±1.20x103/µL, respectively, with statistically significant differences (P<0.05). The highest hematocrit (HTC) value was observed in fish receiving 40 g/kg extract (25.67±6.03%). These results indicate that M. cajuputi leaf extract supplementation in feed can enhance hematological parameters and antibacterial efficacy in carp infected with Aeromonas hydrophila.
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King Mongkut's Agricultural Journal
References
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