Phosphate Solubility and Siderophore Synthesis Properties of Burkholderia caribensis RE230 Involve in Rice Growth Promoting

Abstract

Mutagenesis of plant growth promoting rhizobacteria (PGPR) of rice, Burkholderia caribensis RE230 with EZ-Tn5™ Tnp Transposome™ by electroporation, was investigated. One hundred and six of Tn5 transposon mutants were screened. Four of each Tn5 transposon mutants, which abolished phosphate solubility or siderophore synthesis properties, were selected and tested for plant growth promoting potential on rice (Oryza sativa CV. KDML105) in pot experiment under greenhouse condition. All of mutants gave lower the growth promoting potential than wild type. These were indicated that phosphate solubilization and siderophore production involved in plant growth promoting of B. caribensis RE230. Transposon tagging sites were characterized by sequencing and comparing with the GenBank. Several genes were identified and mutant 085 which deficient on phosphate solubility was tagged on the ATP phosphoribosyltransferase (hisG) gene, that involves transcriptional regulation based primarily on nutrient and environment conditions such as nutrient deficiency and drought condition, was also identified.