Effect of using Suaeda maritima leave extract as a feed additive on the resistance to Vibrio parahaemolyticus and the growth of Pacific white shrimp (Litopenaeus vannamei) during nursing

Abstract

This research aims to study the appropriate types of solvent for the antioxidant substance of
seablite, Suaeda maritime, extract and supplementation of seablite extract in the diet for growth and
resistance to V. parahaemolyticus in Pacific white shrimp larvae. Shrimp diets were mixed with Suaeda
maritima extract at 0, 25, 50 and 75 g / kg diet and cultured for 15 days then be challenged tested for
resistance against V. parahaemolyticus at concentrations of 108 CFU / mL. Total phenolic compounds of
Suaeda maritima extracted by distilled water was highest 8.41±0.02 μg GAE/g followed by ethanol and
petroleum ether with statistical difference (P <0.05). Total flavonoid contents of Suaeda maritima extracted
by petroleum ether showed the highest 47.98±0.12 μg QE/g followed by ethanol and distilled water
(42.89±0.11, 5.60±0.04 μg QE/g), respectively (P <0.05). Survival rates of shrimp fed extracts from the
leaves of Suaeda maritima various formulations did not differ statistically (P> 0.05), but shrimp fed extracts
from the leaves of Suaeda maritima 50 g / kg diet had the best growth and survival rate at 4 days challenged