Partial Cloning and Expression of myo-inositol 1-phosphate synthase(MIPS) Gene in Wild and Cultivated Sugarcane under Mimic Sodic Saline Soil

Abstract

Sodic saline soil is the soil containing high concentration of salts with high pH. It is harmful and affects
to the plant growth. Sugarcane is naturally salt sensitive plant which is affected by sodic saline soil. When the
plant is grown in such soil condition, myo-inositol 1-phosphate synthase (MIPS) gene will be induced to
express. The objectives of this experiment were to partial clone, preliminary characterization of MIPS gene and
to examine the expression levels of MIPS gene in wild and cultivated sugarcane (KPS 94-13) plants subjected
to mimic sodic saline soil condition. Both types of sugarcane plants were grown in 1/10 Hoagland nutrient
solution containing 200 mM NaCl at pH 8 for 0, 12, 24, 48 and 72 hr. The expression of the gene was determined
using real-time PCR. It was found that the expressions of MIPS gene in root of wild and cultivated sugarcane
were highest at 24 and 48 hr after receiving stress condition, respectively. Expressions of the gene in leaves of
both sugarcane species were highest at 72 hr. It was also found that the expressions of the gene in root were
higher than those in leaves. When the expressions of the gene in root and leave of both sugarcane species

were compared it was indicated that the expressions of the gene in wild sugarcane were higher than those
of cultivated sugarcane at all time course of stress that mimics to sodic saline soil condition.