Effect of Kinetin and IAA on Growth and Antioxidants in Bacopa monnieri

Abstract

Brahmi (Bacopa monnieri) is not only aquarium ornamental plant but also is used as folk medicine.
In order to control constant quality, the tissue culture of Brahmi was applied. Semi-solid MS basal medium
supplemented with auxin (indole-3-acetic acid ; IAA) at concentrations of 0, 0.1, 0.2 and 0.3 mg/ L
with combination of cytokinin (furfurylaminopurine; kinetin) at concentrations of 0, 0.5, 1.0 and 1.5 mg/ L
was evaluated. Then, the axenic tissue were cultured on those media. The result showed that medium
supplemented with kinetin 1.5 mg/L could induce maximum height as 80.74 ± 4.10mm. The highest
number of branches of 12.67 ± 0.91 was recorded at the combination of 0.1 mg/L IAA and 0.5 mg/L
kinetin on MS media.The highest fresh weight and dry weight as well occurred on medium
with combination of 0.1 mg/L IAA and 0.5 mg/L kinetin. Subsequently, antioxidant activities were determined
from ethanolic extract by three methods: total phenolic compounds (TPC), DPPH (1,1-diphenyl-2
- picrylhydrazyl radical) and ABTS (2,20azinobis-(3-ethylbenzthiazoline-6-sulfonic acid). The highest
antioxidant activity was recorded at concentration of 0.1 mg/ L IAA combined with 1.5 mg/ L kinetin.