Identification and Expression Pattern of EgBADH2, Betaine aldehyde dehydrogenase in Tenera Oil Palm
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Published:
Mar 31, 2020
Keywords:
water deficit stress responsive gene PEG real-time PCR oil palm
Main Article Content
Abstract
The Betaine aldehyde dehydrogenase (BADH) is a gene controlling the synthesis of an enzyme in the glycine betaine (GB) biosynthesis pathway. GB is an osmoprotectant that is found in higher plants that synthesize and accumulate it in cells in response to drought stress. The objectives of this study were to clone the full-length of the EgBADH2 gene of the Tenera oil palm and to study the expression pattern of this gene in the oil palm under conditions of water deficit. The results indicated that the full length of the EgBADH2 gene was 1,512 bp and
it encoded a protein of 503 amino acid residues. From the phylogenetic tree analysis performed, it was found that the EgBADH2 gene is closely related to the BADH2 gene, which is present in a range of plants including
Cocos nucifera, Phoenix dactylifera, Musa acuminate ‘malaccensis’ and Ananas comosus. The gene expression pattern analysis of 14 days water deficit stressed oil palm plants indicated that the expression levels of the gene after 0, 1 and 2 days of stress were 1±0.04, 0.87±0.07 and 0.74±0.53, respectively, results which were not significantly different. However, significant differences of gene expression levels at the 95% confidence interval were observed for plants subject to between 4 and 14 days of water deficit stress. Therefore, the expression of the EgBADH2 gene was reduced when the oil palm seedlings were subject to water deficit stress for about 14 days.
Article Details
How to Cite
Wichasawasdi, J., Arunyanark, A. ., & Chanprame, S. . (2020). Identification and Expression Pattern of EgBADH2, Betaine aldehyde dehydrogenase in Tenera Oil Palm. King Mongkut’s Agricultural Journal, 38(1), 24–33. retrieved from https://li01.tci-thaijo.org/index.php/agritechjournal/article/view/240985
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Research Articles
King Mongkut's Agricultural Journal
References
บุศริน อิ่มอินทร์, นงลักษณ์ เทียนเสรี และสนธิชัย จันทร์เปรม. 2555. การบ่งชี้ยีนที่ควบคุมการสร้าง betaine aldehyde dehydrogenase
ที่อ้อยใช้ตอบสนองต่อสภาพขาดน้ำ. ใน การประชุมวิชาการแห่งชาติ มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตกำแพงแสน ครั้งที่ 9.
น. 2166-2173.
อัญชนา รอดรังนก, กนกวรรณ เที่ยงธรรม และสนธิชัย จันทร์เปรม. 2560. การจำแนก และรูปแบบการแสดงออกของยีน NIP6;1 (Boric Acid channel for preferential transport of boron) ในปาล์มน้ำมันชนิดเทอเนร่า. ว.วิทย.กษ. 48 (2): 174-185.
Ali, Q., Ehahi, M., Hussain, B., Khan, N. H., Ali, F., and EIahi, F. 2011. Genetic improvement of maize (Zea mays L.) against drought stress: An overview. Agri. Sci. Res. J. 10: 228- 237.
Amudha, J., and Balasubramani, G. 2011. Recent molecular advances to combat abiotic stress tolerance in crop plants.
Biotechnol. Mol. Biol. Rev. 6 (2): 31-58.
Beule, T., Camps, C., Debiesse, S., Tranchant, C., Dussert, S., Sabau, X., Jaligot, E., Alwee, S. S. R. S., and Tregear, J. W. 2011. Transcriptome analysis reveals differentially expressed genes associated with the mantled homeotic flowering abnormality in oil palm (Elaeis guineensis). Tree Genetics & Genomes 7: 169-182.
Blumwald, E. 2000. Sodium transport and salt tolerance in plants. Curr. Opin. Cell Biol. 12: 431- 434.
Bray, E. A., Bailey-Serres, J., and Weretilnyk, E. 2000. Responses to abiotic stresses. pp. 1158- 249. In: W. Gruissem, B. Buchannan, R. Jones, eds. Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists.
Fitzgerald, T. L., Waters, D. L. E., and Henry, R. J. 2009. Betaine aldehyde dehydrogenase in plants. Plant Biol. 11: 119-130.
Hasthanasombut, S., Paisarnwipatpong, N., Triwitayakorn, K., Kirdmanee, C., and Supaibulwatana, K. 2011. Expression of
OsBADH1 gene in Indica rice (Oryza sativa L.) in correlation with salt, plasmolysis, temperature and light stresses.
POJ. 4 (7): 400-407.
Hualkasin, W., Thongin, W., Petsean, K., Phongdara, A., and Nakkaew, A. 2013. Molecular cloning and characterization of the late embryogenesis abundant group 4 (EgLEA4) gene from oil palm (Elaeis guineensis Jacq.). Songklanakarin J. Sci. Technol. 35 (3): 275-285.
Incharoensakdi, A., and Kum-arb, U. 1998. Betaine aldehyde dehydrogenase from a halotolerant cyanobacterium Aphanothece halophytica: purification, properties, and regulation by salinity.1998. J. Sci. Soc. 24: 231-240.
Ishibashi, K. 2006. Aquaporin subfamily with unusual NPA boxes. Biochim Biophys Acta. 1758: 989-993.
Jiang, T., Fountain, J., Davis, G., Kemerait, R., Scully, B., Lee, R. D., and Guo, B. 2012. Root morphology and gene expression analysis in response to drought stress in maize (Zea mays). Plant Mol. Biol. Rep. 30: 360-369.
Laksana, C., and Chanprame, S. 2015. A simple and rapid method for RNA extraction from young and mature leaf of oil palm
(Elaeis guineensis Jacq.). ISSAAS J. 21 (1): 96-106.
Lam, M. K., Tan, K. T., Lee, K. T., and Mohamed, A. R. 2009. Malaysian palm oil: surviving the food versus fuel dispute
for a sustainable future. Renew Sust. Energ Rev.13: 1456-1464.
Li, G., Wu, H., Sun, Y., and Zhang, S. 2016. Betaine aldehyde dehydrogenase (BADH) expression and betaine production in sugarbeet cultivars with different tolerances to drought stress. Sugar Tech. 18 (4): 420-423.
Liu, J., Zeng, H., Li, X., Xu, L., Wang, Y., Tang, W., and Han, L. 2010. Isolation and characterization of the betaine aldehyde dehydrogenase gene in Ophiopogon japonicas. Open Biotechnol J. 4: 18-25.
Lu, Y., Xu, J., Yuan, Z., Hao, Z., Xie, C., Li, X., Shah, T., Lan, H., Zhang, S., Rong, T., and Xu, Y. 2012. Comparative LD mapping using single SNPs and haplotypes identifies QTL for plant height and biomass as secondary traits of drought tolerance
in maize. Mol. Breeding. 30 (1): 407-418.
Mitsuya, S., Yokota, Y., Fujiwara, T., Mori, N., and Takabe, T. 2009. OsBADH1 is possibly involved in acetaldehyde oxidation
in rice plant peroxisomes. FEBS Letters 583: 3625-3629.
Nakamura, T., Yokota, S., Muramoto, Y., Tsutsui, K., Oguri, Y., Fukui, K., and Takabe, T. 1997. Expression of a betaine aldehyde dehydrogenase gene in rice, a glycinebetaine non-accumulator, and possible localization of its protein in peroxisomes. Plant J. 11 (5): 1115-1120.
Oishi, H., and Ebina, M. 2005. Isolation of cDNA and enzymatic properties of betaine aldehyde dehydrogenase from Zoysia tenuifolia. J. Plant Physiol. 162: 1077-1086.
Parmentier-Line, C. M., Panta, G. R., and Rowland, L. J. 2002. Changes in dehydrin expression associated with cold,
ABA and PEG treatments in blueberry cell cultures. Plant Sci. 162: 273-282.
Rival, A., and Jaligot, E. 2010. Oil palm biotechnologies are definitely out of infancy. O.C.L.17 (6): 368-374.
Rodriguez, M., Canales, E., Borroto, C. J., Carmona, E., Lopez, J., Pujol, M., and Borras-Hidalgo, O. 2006. Identification of genes induced upon water-deficit stress in a drought-tolerant rice cultivar. J. Plant Physiol. 163 (5): 577-584.
Shivaraj, S. M., Deshmukh, R. K., Rai, R., Belanger, R., Agrawal, P. K., and Dash, P. K. 2017. Genome-wide identification, characterization, and expression profile of aquaporin gene family in Flax (Linum usitatissimum). Sci. Rep. 17 p.
Sithtisarn, S., Harinasut, P., Pornbunlualap, S., and Cha-um, S. 2009. Accumulation of glycinebetaine and betaine aldehyde dehydrogenase activity in Eucalyptus camaldulensis clone T5 under in vitro salt stresss. Kasetsart J. (Nat Sci.) 43 (5):
146-152.
Sun, C. X., Cao, H. X., Shao, H. B., Lei, W. T., and Xiao, Y. 2011. Growth and physiological responses to water and nutrient stress
in oil palm. Afr. J. Biotech. 10 (51): 10465-10471.
Wang, F. W., Wang, M. L., Guo, C., Wang, N., Li, X. W., Chen, H., Dong, Y. Y., Chen, X. F., Wang, Z. M., and Li, H.Y. 2016.
Cloning and characterization of a novel betaine aldehyde dehydrogenase gene from Suaeda corniculata.
Genet. Mol. Res. 15 (2): 14.
Yu, X., Bai, G., Liu, S., Luo, N., Wang, Y., Richmond, D. S., Pijut, P. M., Jackson, S. A., Yu, J., and Jiang, Y. 2013. Association of candidate genes with drought tolerance traits in diverse perennial ryegrass accessions. J. Exp. Bot. 64 (6): 1537-1551.
Zhang, L., Gao, M., Hu, J., Zhang, X., Wang, K., and Ashraf, M. 2012. Modulation role of abscisic acid (ABA) on growth,
water relations and glycine betaine metabolism in two maize (Zea mays L.) cultivars under drought stress.
Int. J. Mol. Sci. 13: 3189-3202.
ที่อ้อยใช้ตอบสนองต่อสภาพขาดน้ำ. ใน การประชุมวิชาการแห่งชาติ มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตกำแพงแสน ครั้งที่ 9.
น. 2166-2173.
อัญชนา รอดรังนก, กนกวรรณ เที่ยงธรรม และสนธิชัย จันทร์เปรม. 2560. การจำแนก และรูปแบบการแสดงออกของยีน NIP6;1 (Boric Acid channel for preferential transport of boron) ในปาล์มน้ำมันชนิดเทอเนร่า. ว.วิทย.กษ. 48 (2): 174-185.
Ali, Q., Ehahi, M., Hussain, B., Khan, N. H., Ali, F., and EIahi, F. 2011. Genetic improvement of maize (Zea mays L.) against drought stress: An overview. Agri. Sci. Res. J. 10: 228- 237.
Amudha, J., and Balasubramani, G. 2011. Recent molecular advances to combat abiotic stress tolerance in crop plants.
Biotechnol. Mol. Biol. Rev. 6 (2): 31-58.
Beule, T., Camps, C., Debiesse, S., Tranchant, C., Dussert, S., Sabau, X., Jaligot, E., Alwee, S. S. R. S., and Tregear, J. W. 2011. Transcriptome analysis reveals differentially expressed genes associated with the mantled homeotic flowering abnormality in oil palm (Elaeis guineensis). Tree Genetics & Genomes 7: 169-182.
Blumwald, E. 2000. Sodium transport and salt tolerance in plants. Curr. Opin. Cell Biol. 12: 431- 434.
Bray, E. A., Bailey-Serres, J., and Weretilnyk, E. 2000. Responses to abiotic stresses. pp. 1158- 249. In: W. Gruissem, B. Buchannan, R. Jones, eds. Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists.
Fitzgerald, T. L., Waters, D. L. E., and Henry, R. J. 2009. Betaine aldehyde dehydrogenase in plants. Plant Biol. 11: 119-130.
Hasthanasombut, S., Paisarnwipatpong, N., Triwitayakorn, K., Kirdmanee, C., and Supaibulwatana, K. 2011. Expression of
OsBADH1 gene in Indica rice (Oryza sativa L.) in correlation with salt, plasmolysis, temperature and light stresses.
POJ. 4 (7): 400-407.
Hualkasin, W., Thongin, W., Petsean, K., Phongdara, A., and Nakkaew, A. 2013. Molecular cloning and characterization of the late embryogenesis abundant group 4 (EgLEA4) gene from oil palm (Elaeis guineensis Jacq.). Songklanakarin J. Sci. Technol. 35 (3): 275-285.
Incharoensakdi, A., and Kum-arb, U. 1998. Betaine aldehyde dehydrogenase from a halotolerant cyanobacterium Aphanothece halophytica: purification, properties, and regulation by salinity.1998. J. Sci. Soc. 24: 231-240.
Ishibashi, K. 2006. Aquaporin subfamily with unusual NPA boxes. Biochim Biophys Acta. 1758: 989-993.
Jiang, T., Fountain, J., Davis, G., Kemerait, R., Scully, B., Lee, R. D., and Guo, B. 2012. Root morphology and gene expression analysis in response to drought stress in maize (Zea mays). Plant Mol. Biol. Rep. 30: 360-369.
Laksana, C., and Chanprame, S. 2015. A simple and rapid method for RNA extraction from young and mature leaf of oil palm
(Elaeis guineensis Jacq.). ISSAAS J. 21 (1): 96-106.
Lam, M. K., Tan, K. T., Lee, K. T., and Mohamed, A. R. 2009. Malaysian palm oil: surviving the food versus fuel dispute
for a sustainable future. Renew Sust. Energ Rev.13: 1456-1464.
Li, G., Wu, H., Sun, Y., and Zhang, S. 2016. Betaine aldehyde dehydrogenase (BADH) expression and betaine production in sugarbeet cultivars with different tolerances to drought stress. Sugar Tech. 18 (4): 420-423.
Liu, J., Zeng, H., Li, X., Xu, L., Wang, Y., Tang, W., and Han, L. 2010. Isolation and characterization of the betaine aldehyde dehydrogenase gene in Ophiopogon japonicas. Open Biotechnol J. 4: 18-25.
Lu, Y., Xu, J., Yuan, Z., Hao, Z., Xie, C., Li, X., Shah, T., Lan, H., Zhang, S., Rong, T., and Xu, Y. 2012. Comparative LD mapping using single SNPs and haplotypes identifies QTL for plant height and biomass as secondary traits of drought tolerance
in maize. Mol. Breeding. 30 (1): 407-418.
Mitsuya, S., Yokota, Y., Fujiwara, T., Mori, N., and Takabe, T. 2009. OsBADH1 is possibly involved in acetaldehyde oxidation
in rice plant peroxisomes. FEBS Letters 583: 3625-3629.
Nakamura, T., Yokota, S., Muramoto, Y., Tsutsui, K., Oguri, Y., Fukui, K., and Takabe, T. 1997. Expression of a betaine aldehyde dehydrogenase gene in rice, a glycinebetaine non-accumulator, and possible localization of its protein in peroxisomes. Plant J. 11 (5): 1115-1120.
Oishi, H., and Ebina, M. 2005. Isolation of cDNA and enzymatic properties of betaine aldehyde dehydrogenase from Zoysia tenuifolia. J. Plant Physiol. 162: 1077-1086.
Parmentier-Line, C. M., Panta, G. R., and Rowland, L. J. 2002. Changes in dehydrin expression associated with cold,
ABA and PEG treatments in blueberry cell cultures. Plant Sci. 162: 273-282.
Rival, A., and Jaligot, E. 2010. Oil palm biotechnologies are definitely out of infancy. O.C.L.17 (6): 368-374.
Rodriguez, M., Canales, E., Borroto, C. J., Carmona, E., Lopez, J., Pujol, M., and Borras-Hidalgo, O. 2006. Identification of genes induced upon water-deficit stress in a drought-tolerant rice cultivar. J. Plant Physiol. 163 (5): 577-584.
Shivaraj, S. M., Deshmukh, R. K., Rai, R., Belanger, R., Agrawal, P. K., and Dash, P. K. 2017. Genome-wide identification, characterization, and expression profile of aquaporin gene family in Flax (Linum usitatissimum). Sci. Rep. 17 p.
Sithtisarn, S., Harinasut, P., Pornbunlualap, S., and Cha-um, S. 2009. Accumulation of glycinebetaine and betaine aldehyde dehydrogenase activity in Eucalyptus camaldulensis clone T5 under in vitro salt stresss. Kasetsart J. (Nat Sci.) 43 (5):
146-152.
Sun, C. X., Cao, H. X., Shao, H. B., Lei, W. T., and Xiao, Y. 2011. Growth and physiological responses to water and nutrient stress
in oil palm. Afr. J. Biotech. 10 (51): 10465-10471.
Wang, F. W., Wang, M. L., Guo, C., Wang, N., Li, X. W., Chen, H., Dong, Y. Y., Chen, X. F., Wang, Z. M., and Li, H.Y. 2016.
Cloning and characterization of a novel betaine aldehyde dehydrogenase gene from Suaeda corniculata.
Genet. Mol. Res. 15 (2): 14.
Yu, X., Bai, G., Liu, S., Luo, N., Wang, Y., Richmond, D. S., Pijut, P. M., Jackson, S. A., Yu, J., and Jiang, Y. 2013. Association of candidate genes with drought tolerance traits in diverse perennial ryegrass accessions. J. Exp. Bot. 64 (6): 1537-1551.
Zhang, L., Gao, M., Hu, J., Zhang, X., Wang, K., and Ashraf, M. 2012. Modulation role of abscisic acid (ABA) on growth,
water relations and glycine betaine metabolism in two maize (Zea mays L.) cultivars under drought stress.
Int. J. Mol. Sci. 13: 3189-3202.