Expression Profiling of Cutinase and Endopolygalacturonic-related Genes in <I>Colletotrichum capsici</I> During Chili Fruit Infection
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Abstract
This study determined the expression level of cutinase (CUT) and endopolygalacturonase (endo-PG) genes of Colletotrichum capsici after inoculating on green and red chili fruit stages of Bang Chang variety by real-time PCR technique. The inoculated green chili fruits were sampled at 0, 24, 48, 72, 96, 120, 192 and 240 hours after inoculation (HAI) and red chili fruits were sampled at 0, 24, 48, 72, 96, 120 and 144 HAI. It showed that CUT was the greatest up-regulated at 72 hours after inoculation (HAI) on red fruit stage and at 240 HAI on green fruit stage. The maximum expression of endo-PG was observed at 144 and 192 HAI on red and green fruits, respectively. Disease symptom started to produce at 144 HAI on red chili fruit and 240 HAI on green fruit.
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