Broad - spectrum Antimicrobial Compounds from <I>Streptomyces coerulescens </I> NN91
Article Sidebar
Main Article Content
Abstract
This research aims to investigate the compounds from Streptomyces coerulescens isolate NN91 which showed the good activity against Candida albicans, Micrococcus luteus, and Staphylococcus aureus. The cultivation of this strain in ISP2 in shake flask at room temperature (28 ºC) showed the bioactive compound activity at the maximum value at day 7 against test organisms and exhibited thermal stabilities at 100 ºC for 15 minutes. Following supernatant extraction, crude extract showed a minimal inhibitory concentration (MIC) with M. luteus and S. aureus by 0.039 and 0.009 µg / ml, respectively. Molecular identification by sequencing of 16S rRNA gene revealed that the actinomycete isolate NN91 was similarity to S. coerulescens (AB184122) with 98.9 %. This result can be applied for drug development and broad - spectrum susceptible treatment in near future.
Article Details
References
Alexander, M. 1977. Introduction to Soil Microbiology. 2nd ed. John Wiley & Sons, New York. 467 p.
Arasu, M.V., V. Duraipandiyan, P. Agastian and S. Ignacimuthu. 2008. Antimicrobial activity of Streptomyces spp. ERI-26 recovered from Western Ghats of Tamil Nadu. Journal de Mycologie Médicale 18(3): 147-153.
Atta, H.M., S.M. Dabour and S.G. Desoukey. 2009. Sparsomycin antibiotic production by Streptomyces sp. AZ-NIOFD1: Taxonomy, fermentation, purification and biological activities. American-Eurasian Journal of Agriculture and Environment Science 5(3): 368-377.
Boudjella, H., K. Bouti, A. Zitouni, F. Mathieu, A. Lebrihi and N. Sabaou. 2006. Taxonomy and chemical characterization of antibiotics of Streptosporangium Sg 10 isolated from a Saharan soil. Microbiological Research 161(4): 288-298.
Clark, G.J., D. Langley and M.E. Bushell. 1995. Oxygen limitation can induce microbial secondary metabolite formation: investigations with miniature electrodes in shaker and bioreactor culture. Microbiology 141: 663-669.
Kieser, T., M.J, Bibb, M.J. Buttner, K.F. Chater and D.A. Hopwood. 2000. Practical Streptomyces Genetics. John Innes Foundation, Norwich. 613p.
Lazzarini A., L. Cavaletti, G. Toppo and F. Marinelli. 2000. Rare genera of actinomycetes as potential producers of new antibiotics. Antonie Van Leeuwenhoek 78(3): 399-405.
Lyons, A.J. and T.G. Pridham. 1971. Streptomyces torulosus sp. n., an unusual knobby-spored Taxon. Applied Microbiology 22(2): 190-193.
Maleki, H., A. Dehnad, S. Hanifian and S. Khani. 2013. Isolation and molecular identification of Streptomyces spp. with antibacterial activity from northwest of Iran. BioImpacts 3(3): 129-134.
Niyomvong, N., W. Pathom-aree, A. Thamchaipenet and K. Duangmal. 2012. Actinomycetes from tropical limestone caves. Chiang Mai Journal of Science 39(3): 373-388.
Narayana, K.J.P. and M. Vijayalakshmi. 2009. Chitinase Production by Streptomyces sp. ANU 6277. Brazilian Journal of Microbiology 40(4): 725-733.
Okami, Y. and K. Hotta. 1988. Search and discovery of new antibiotics. pp. 33-67. In: M. Goodfellow, S.T. Williams and M. Mordarski (eds.). Actinomycetes in Biotechnology. Academic Press, London
Olano, C., C. Mendez and J.A. Salas. 2009. Antitumor compounds from marine actinomycetes. Marine Drugs 7(2): 210-248.
Sasaki, T., T. Otani, K. Yoshida, N. Unemi, M. Hamada and T. Takeuchi. 1997. Discovery of methyl-2-(2'-hydroxyphenyl)-2-oxazoline-4-carboxylate as a secondary metabolite from Actinomadura sp. Journal of Antibiotics 50(10): 881-883.
Schumacher, R.W., B.L. Harrigan and B.S. Davidson. 2001. Kahakamides A and B, new neosidomycin metabolites from a marine-derived actinomycete. Tetrahedron Letters 42(31): 5133-5135.
Selvin, J., S. Joseph, K.R.T. Asha, W.A. Manjusha, V.S. Sangeetha, D.M. Jayaseema, M.C. Antony and A.L. Denslin Vinitha. 2004. Antibacterial potential of antagonistic Streptomyces sp. isolated from marine sponge Dendrilla nigra. FEMS Microbiology Ecology 50(2): 117-122.
Sengupta, S., A. Pramanik, A. Ghosh and M. Bhattacharyya. 2015. Antimicrobial
activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem. BMC microbiology 15(1): 170, doi: 10.1186/s12866-015-0495-4
Siddiqi, S.H., L.B. Heifets, M.H. Cynamon, N.M. Hooper, A. Laszlo, J.P. Libonati, P.J. Lindholm-Levy and N. Pearson. 1993. Rapid broth macrodilution method for determination of MICs for Mycobacterium avium isolates. Journal of Clinical Microbiology 31(9): 2332-2338.
