Culture Technique for Frog-breeder (Hoplobatrachus rugulosus) to Prevent Bacteria (Mycobacterium spp.) Infection on Internal Organs

Abstract

The objective of this study aims to reduce Mycobacterium spp. infection on intimal organs of the frog-breeder. The experimental trials were triplicate divided into five groups, T1: Dry rounded cement tank, T2: Rounded cement tank with water 60:40, T3: Dry squared cement tank, T4: cage on land with water, T5: Floating cage in concrete tank. All experimental group were challenged in the Mycobacterium spp. dissemination place. The frog-breeders were stocked at fifteen frogs per square meter. The frog-breeders; the overall weight percentages increase of flog broodstock in the different rearing type were 0.00±20.99, 44.25±56.34, 0.26±5.16, 20.00±6.78 and 16.23±8.10, respectively. There was a significant difference in weight gain of group 1 compared to group 2, 4 and 5 (p<0.05). The survival rate of frog-breeder in the different environments were 90.63±6.25, 100±0.00, 93.47±7.53, 97.91±1.39 and 97.91±1.39%, respectively. The highest survival rate was observed in group which frog-reared in the half-water rounded cement tank (Group 2) and it had a significant difference from reared groups 1 and 3 (p<0.05). The biopsy results, there was no detected of Mycobacterium spp. In the group 2 by PCR method. Furthermore, the frog tadpoles of the frog breeding in group 2 were showed a normally growth rate.