ความหลากหลายของชุมชนแบคทีเรียจากห้อมใบใหญ่ (Strobilanthes cusia)  และห้อมใบเล็ก (Strobilanthes sp.) โดยการวิเคราะห์เมตาจีโนมิกส์

Nuttaporn Chanchay; Piyawan Sangthong; Ansaya Boonprajaub

doi:10.14456/jare-mju.2026.29

Authors

Nuttaporn Chanchay Department Agro-industrial Biotechnology, Maejo University Phrae Campus, Phare https://orcid.org/0009-0009-9323-8299
Piyawan Sangthong Department Agro-industrial Biotechnology, Maejo University Phrae Campus, Phare
Ansaya Boonprajaub Department Agro-industrial Biotechnology, Maejo University Phrae Campus, Phare

DOI:

https://doi.org/10.14456/jare-mju.2026.29

Keywords:

Strobilanthes cusia, Strobilanthes sp., DNA purity, metagenomics, genetic diversity

Abstract

Metagenomics is an effective tool for analyzing the composition and diversity of microbial communities in the environment without the need for cultivation, making it suitable for studying plant-associated microorganisms. Strobilanthes spp. (indigo plant) is an economically and scientifically important medicinal plant in northern Thailand. Its leaves are used to produce natural indigo dye and contain bioactive compounds with potential pharmaceutical applications. Understanding the microbial communities associated with Strobilanthes spp. is essential for elucidating their roles in promoting plant growth and synthesizing secondary metabolites. This study aimed to investigate the bacterial community diversity in leaves of S. cusia (large-leaf) and Strobilanthes sp. (small-leaf) collected from five sites in Phrae province using 16S rRNA gene sequencing on the illumina platform. The bacterial communities were predominantly affiliated with the phylum Proteobacteria, particularly the class Gammaproteobacteria and the order Pseudomonadales, including Pseudomonas spp. and unclassified bacteria. These taxa are known to contribute to plant growth promotion and the biosynthesis of bioactive compounds. The findings provide insight into the microbial diversity associated with S. cusia, offering potential applications in the pharmaceutical, textile, and agricultural industries.

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