Effect of disinfectants and plant growth regulator in aquatic plant Bucephalandra sp. micropropagation

Abstract

Bucephalandra spp. is a new type of aquatic plants which found in Borneo. These aquatic plants
have bright spot, known as pearl on the leaves. As this aquatic plant species is very expensive,
slow propagation and imported from abroad. The propagation technique through tissue culture would
hypothesis help to solve this problem. Four experiments were conducted to find out the optimum conditions
for Bucephalandra sp. growth. The first experiment aimed to determine the optimum kind and
concentration of surface sterilization method. The result showed that using 10% NaOCl for 20 minutes
followed by 0.1% HgCl2
for 10 minutes was the best result. After 4 weeks of culturing on MS medium, it
provided the highest of survival (90%) and non-contamination. The second experiment aimed to determine

the optimum concentration of growth regulators. The axenic tissue were cultured using the combination of
adenine sulfate (Ads) at 0, 25, 50, 75 mg/L and 6-benzylaminopurine (BAP) at 0, 0.5, 1.0, 1.5 mg/L
supplemented in MS semi-solid medium. The media contain BAP 0.5 mg/L showed the increase of
plantlets (2.45±0.15 shoot/explant) and new leaves (15.25±0.92) significantly (P<0.05). The third experiment
attempted to evaluate the optimum concentration of NAA (0, 0.1, 0.3, 0.5 and 0.7 mg/L) to induce roots.
After 8 weeks, the MS semi-solid medium with no NAA showed the best increment of roots (8.45±0.67).